M of total extraction technology of flavonoids in the herb extraction equipment research
M of total extraction technology of flavonoids in the herb extraction equipment study atmospheric water decoction technique for extraction of total flavonoids in the herb.
Prevention law 'the material liquid ratio, temperature decoction and decoction time as the main factors in the orthogonal experiment, to determine the best extraction technology of total flavonoids.
Mouth 'herb optimum extraction conditions of total flavonoids in boiling for material liquid than 5 g/L, 75 min, decoction time under the condition of the flavonoids yield is about 0.
For subsequent herbal beverage product research and development to provide the related information.
in recent years, with the improvement of living standard, people care more and more attention to the understanding of health also gradually tend to be more rational.
Thus, promote the some based on the development of herbal health care beverage production enterprises.
Herbs contain all kinds of biological active ingredients, such as flavonoids, total soap, all kinds of active polysaccharides and some antioxidants, etc.
At present, for all kinds of the extraction of active components in the herbs are mainly water decoction method, supercritical fluid extraction, ultrafiltration membrane separation, macroporous adsorption resin purification technology, ultrasonic extraction and microwave extraction technology, etc.
Water decoction method is the most traditional extraction methods, it is to join the right amount of water in the herbs, and then heated to a certain temperature and keep a certain time to filter out boiled liquid method.
This method is not only simple but also scrambled out of the most effective component, is the most commonly used to extract one method of active ingredient in the herb.
The author provided in a company of herbaceous plants as raw material, study of water decoction method to extract the flavones process, in order to the follow-up research and development of herbal drinks provide related information.
total extraction technology of flavonoids in the herb.
Process as shown in figure 1.
the specific steps are: take a small packaging plant, an average of about 170 g, with clear water is rinsed repeatedly 3 times, remove surface impurities and dust, etc.
According to the material liquid than 1:5 g a few water, heated to boiling and maintain 75 min, make the maximum total flavonoids of herbaceous plants leaching.
Decoction, after the first 6 layer of gauze is adopted to improve the coarse filter, filter out the larger herb residue, the filtrate obtained with 5, 000 r/min, high-speed centrifuge centrifuge for 10 min, separation to remove small residue, finally clarify extract.
Determination of the flavonoids content in the extract.
the flavonoids content determination.
rutin reference substance solution preparation.
Precision according to take 120 rutin 50 ℃ decompression drying to constant weight.
5 mg, put in 250 ml volumetric flask, add methanol solution, water bath on micro heat to dissolve, cooling, add methanol to scale, shake well, that is 0.
202 mg/ml rutin reference substance solution.
the choice of measurement wavelength.
precision from rutin reference substance solution and herb extract each 4 ml, put in 25 ml volumetric flask, plus 5% NaNO2 solution 1 ml, shake well, 6 min, add Al (
3 solution 1 ml, shake well, 6 min, 1 ml, and 4% NaOH solution and diluted to scale, shake well, after 15 min 1 cm than the color of plating, accompanying reagent for contrast blank, in 300 ~ 600 nm scanning, find the maximum absorption wavelength.
drawing standard curve.
Precision absorb rutin reference substance solution 0, 1.
0 to 3.
0 ml put in 25 ml volumetric flask,.
With concentration (
With the absorbance value (
Linear regression, the linear regression equation and correlation coefficient.
total content of flavonoids in the sample determination.
Precision learn herb extract 4 ml in 25 ml volumetric flask and parallel operation three times, meanwhile set up blank control.
in order to determine the best extraction technology of total flavonoids, to liquid ratio, temperature decoction and decoction time as the main factors in the orthogonal experiment.
Orthogonal test table to1 factor level.
4 flavonoids yield calculation.
According to the following formula: flavonoids yield = (
Extract content of total flavonoids in/plant to the quality of the sample)
X 100% rutin standard curve drawn by the spectrum scanning maximum absorption wavelength of 510 nm, 510 nm in the determination of rutin solution's absorbance value under different concentration, and drawing standard curve, as shown in figure 2.
by figure 2 linear regression equation A = 12.
369 c -
1191, the correlation coefficient (
In 924, five curve 0.
01 ~ 0.
5 mg/ml has good linear relationship.
the best extraction process of determining the table 2 shows that three factors influence on flavonoids extraction progression for decoction temperature> solid-liquid ratio> decoction time, at the same time, it is concluded that the best process for the material liquid than 1:7 g/L, boiling water, decoction time, 75 min, the total content of flavonoids in the extracts obtained at this time of 1 280 mg.
Also can be seen from table 2, the material liquid ratio can also be selected as 1 g/L, under the condition of the total content of flavonoids in the extracts of 1 247 mg, although slightly lower yield, but can greatly reduce the production cost for the enterprise.
Therefore, ultimately determine the extraction technology of total flavonoids of material liquid than 5 g/L, boiling water, decoction time, 75 min, the flavonoid yield is about 0.
3 test using herbs as raw materials, the traditional water decoction method to extract total flavonoids of bioactive substances, and combining with actual production it is concluded that the best extraction technology conditions: material liquid than 5 g/L, boiling water, decoction time, 75 min, under the condition of the flavonoid yield is about 0.
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