Extraction equipment used in enzyme of aqueous two-phase extraction
Extraction equipment in the aqueous two-phase extraction of enzyme application can effectively remove cellular debris, and at the same time can also be required for the purification of initial solute.
the existence of cellular debris, compared to a significant change, density increased, cell debris often preferred allocation in the next phase, thus make the next phase viscosity increases, the average more than 95% of the enzyme by extracting equipment to the rich in polyethylene glycol (peg) phase, while the insolubles and soluble protein and other impurities in the next phase.
But the enzyme is not must be distributed in the upper.
as a result of the pure enzyme is very expensive, as into a polymer to recall is not economic.
However extraction equipment in the aqueous two-phase extraction of enzyme application can solve this problem, very good use of cheaper crude enzyme to a certain degree of hydrolysis, although hydrolysis can reduce viscosity, molecular weight but still produce large molecules within a certain range.
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