Extraction of Chinese catalpa spirit with centrifugal extraction machine
Chinese catalpa spirit element is one kind yellow ketone, is Chinese catalpa tree endophytic fungi in the fermented liquid of flavonoids, secondary metabolites under the condition of pH value is neutral, with organic solvents methanol, ethanol, chloroform, dichloromethane, benzene, ethyl acetate and petroleum ether processing fermented liquid effect is good;
In neutral and weak alkali conditions, Chinese catalpa spirit in the fermented liquid composition is very stable, but in the oil-water two phase extraction when almost all kept in the water phase, difficult to achieve extraction separation.
So when extraction separation, Chinese catalpa spirit need to join the acid, alkali, adjust the ionization balance, change the polarity and oil-water two phase distribution coefficient, leading to more effective for Chinese catalpa spirit element extraction separation.
in the Chinese catalpa spirit element extraction, Chinese catalpa tree of the endophytic fungi with solvent for fermentation, access to Chinese catalpa spirit fermented liquid, and then the need for screening of extractant.
The conventional organic solvents are methanol, ethanol, ethyl acetate, chloroform, dichloromethane, acetone, toluene, deionized water, etc.
Through the same test data comparison, ethyl acetate as extraction solvent effect is best.
in the extraction
Reverse extraction process, with the pH value increases, Chinese catalpa spirit in oil-water two phase distribution ratio decrease.
In the pH = 4.
5, fermented liquid compared with ethyl acetate is 1:1, Chinese catalpa spirit has the highest extraction rate.
specific operation process, will Chinese catalpa spirit, fermented liquid and ethyl acetate according to 1:1 proportion into a centrifugal extraction machine, with the aid of the rotation of the drum, through the turbine disc and the impeller to make two phase mixing and dispersing quickly, two phase solution fully mass transfer, mixing and mass transfer process.
Mixture again after the effect of drum centrifugal force through two separate out of the body side by side.
Chinese catalpa spirit by adding acid and alkali, can adjust ionization balance, change its polarity, so as to realize Chinese catalpa spirit in oil-water two phase transfer, complete Chinese catalpa spirit element extraction separation.
related extraction equipment figure:
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