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In the � � � handle the separation and purification of astragalus polysaccharides extraction technology

by:Deyuan      2020-09-03
The separation and purification of Chinese medicine extraction technology of astragalus polysaccharides
the structure of polysaccharide and protein and nucleic acid are similar, can be divided into primary, secondary, tertiary and quaternary structure. Primary structure including sugar base composition, sequence, sugar chain branch, the location and length of branch. Polysaccharide skeleton by hydrogen bonding between various polymer is formed by the secondary structure, in addition to this and the existence of the tertiary and quaternary structure, it shows that the structure of the polysaccharide is very complex, on the uniform components purified polysaccharide, needs the combination of a variety of methods.
polysaccharides are important biological active substances in plants and animals, all plants and animals have polysaccharide, so to speak. A growing number of studies have found that polysaccharides has a great value to human body, many traditional Chinese medicines such as ginseng, astragalus, angelica sinensis, fructus schisandrae, selfheal, phragmites communis, ganoderma lucidum, etc. , there are many high nutritional value of edible fungi such as mushrooms, hericium, needle mushroom, etc. Polysaccharide purified method are many, the nature of the polysaccharide is the foundation of its purification [ 76 ', introduces the following several kinds of commonly used methods. The separation and purification of Chinese medicine extraction technology of astragalus polysaccharides
1. Grading precipitation
use different polysaccharide molecular size and the solubility and separation, the commonly used a quarter of money salt precipitation method and organic solvent precipitation. Such as export small umbrella purification method of crude polysaccharide, polysaccharide in solution with different concentration of ethanol solution, get more polysaccharide; Asparagus cold water immersion extraction of polysaccharide in one of three ways using acetone fractional precipitation; Tin stone polysaccharide solution used in the desert of the hexadecyl trimethylamine ( CTAB) Complexing refining.
    2. Gel column chromatography,
the commonly used gel with dextran gel ( 交联葡聚糖) And agarose gel ( 琼脂糖) To different concentrations of salt solution and buffer solution as eluent, so that different sizes of polysaccharide molecules get purification, but is not suitable for the separation of glycosaminoglycan. General student extracts for isolating multi-purpose Sepharose, DEAEToyopearl, Sephacryl, Sephadex delicate and get all kinds of polysaccharide. Such as ganoderma lucidum polysaccharide, polysaccharide, polysaccharide extracted by lateral root of aconite, yam polysaccharide, etc.
    3. Cellulose anion exchanger column chromatography commonly used exchanger for
DEAE cellulose and ECTEOLA cellulose, classification of borax type and alkali type two kinds, eluent of different concentration of alkali solution, borax solution available, salt solution, its advantages can be adsorbed impurities, purified polysaccharide, and is suitable for the separation of various medium and acidic polysaccharides and mucopolysaccharide. Such as lily polysaccharide, coastal glehnia root polysaccharide, pseudostellaria root polysaccharide, etc. The separation and purification of Chinese medicine extraction technology of astragalus polysaccharides
4. Other methods
dialysis, ultrafiltration and overspeed centrifugal choose different specifications of the ultrafiltration membrane and ultrafiltration, dialysis and dialysis bag ultracentrifuges operate under certain conditions, according to the molecular size of polysaccharide sample classification, ultrafiltration and dialysis is more often used to remove small molecules. Zone electrophoresis mainly according to the charge properties of polysaccharides of different classification, commonly used have polypropylene phthalic amide gel electrophoresis, cellulose acetate membrane electrophoresis.
5。 1 astragalus polysaccharide extraction and purification of
astragalus polysaccharide is an important effective components in radix astragali, polymer for glucose and arabinose, molecular weight below twenty thousand. Its main effect is to improve the body's immunity, humoral immunity, cellular immunity, the nonspecific immunity, etc. It can make animals increase the spleen, spleen in plasma cell proliferation, promote the synthesis of antibodies, and to combat the effects of prednisone and other immunosuppressants. The separation and purification of Chinese medicine extraction technology of astragalus polysaccharides
access to relevant data, the separation and purification of astragalus polysaccharides of water extract-alcohol precipitation using more, this experiment adopted astragalus polysaccharides crude products. Alcohol sinking method is the most widely used a refined method and its principle is the traditional Chinese medicine effective component is soluble in water and soluble in alcohol, extract and water often contains mucus quality, gelatinized starch, pectin, gum, protein, etc. , with appropriate concentration of ethanol precipitation can be effectively removed. Such as liquid alcohol content of 60% can be removed in the starch, alcohol content of 70%, in addition to whatever a few inactive ingredients such as qualitative, water-soluble pigment, most are precipitation to remove impurities. Due to this method, simple operation, technology and equipment requirements is not high, therefore, since the early seventy s has been in use today, become the 'legal' refined method.
5。
2 of astragalus polysaccharides purification according to the nature of the astragalus polysaccharides hardly soluble in strong ethanol precipitation method can be used to extract and purification. As shown in figure 5 - specific preparation process 1, after the enrichment and centrifugal separation of extract, join the anhydrous ethanol, according to reach for the amount of ethanol concentration of 70% ( vol%) Is ok. Liquid precipitation stand for more than 24 h, then the centrifugal separation and filtration, precipitation with ethanol and acetone washing many times, dry raw astragalus polysaccharides. The separation and purification of Chinese medicine extraction technology of astragalus polysaccharides




this article, focusing on the extraction and purification of astragalus polysaccharides was studied, found with high-pressure extraction technology, astragalus polysaccharides of subsequent separation less impurity, easier to separation and purification, obtain satisfactory result, but this is only a preliminary, we get the raw polysaccharide, get further purification is tasted, if you need in addition to fat, in addition to small molecules such as proteins, in addition to the pigment and oligosaccharides impurities such as operation. The separation and purification of Chinese medicine extraction technology of astragalus polysaccharides
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