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Solvent extraction method of extraction of pomegranate peel polyphenols extraction process

by:Deyuan      2020-09-05
1. Pomegranate peel polyphenols extraction method
pomegranate peel polyphenols extraction method of general with solvent extraction method, alkaline dilute alcohol or alkaline water extraction, ultrasonic assisted extraction, microwave extraction and supercritical CO2 extraction, the extraction process of polyphenols in the choice of appropriate extraction method will help to enhance the efficiency of extraction and extraction effect is enhanced, so as to get ideal experiment effect, create a favorable condition for follow-up study.


solvent extraction solvent extraction method is according to the components in plants in the nature of the dissolved in the solvent, selection of active ingredient solubility is big, does not need to dissolve the solubility of solvent, the composition of the active ingredient from plant tissues dissolved method at present, the polyphenol extraction method is mainly organic solvent extraction method, commonly used with methanol, ethanol, acetone, ethyl acetate, and so on, these organic solvents of polyphenols have good dissolving ability, does not react with polyphenols, and leaching of low impurity, easy separation. By the volume fraction of 20% ethanol as solvent, material liquid than 1:20, under the condition of 50 ℃ extract 1 h, polyphenols yield up to 22. 86%, and achieved good results; Using 50% ethanol as extraction solvent in the liquid than 25:1; seven extract 1 ℃ condition. 5 h, pomegranate peel polyphenols yield is 16. 28%; Through an experiment with methanol, ethanol, ethyl acetate and water, respectively in xinjiang pomegranate peel total polyphenol extraction, the polyphenols yield is methanol > ethanol > water > ethyl acetate. Because of methanol and ethanol as solvent polyphenols yield were similar, and three kinds of solvents ethanol is of high safety, low price, wide sources, high recovery rate, so choose ethanol as extraction solvent. Thus, the extraction of pomegranate peel polyphenols on the selection of extraction solvent, ethanol is popular.
pomegranate for plants in deciduous shrubs or small trees, native to central Asia, to the western han dynasty zhang qian to the western regions is introduced into our country, our country far lintong zaozhuang, shandong, anhui, shaanxi, sichuan huili, yunnan mengzi has grown. Pomegranate skin, the dry fruit of pomegranate and its acid, bitter.
pomegranate peel polyphenols is the floorboard of the hydroxyl compounds in pomegranate peel, including ellagic tannins, gallic tannin, ellagic acid, gallic acid, catechin, flower pigment, chlorogenic acid, ferulic acid and quercetin and other compounds, accounted for 10% ~ 20% of dry weight.
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