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The progress of Chinese medicine extraction and separation technology of high and new technology application

by:Deyuan      2020-09-03
The progress of traditional Chinese medicine (TCM) in the process of extraction and separation of high and new technology application new adsorbent electrophoresis (
电泳) And chromatography ( 色谱法) The application of separation technology
new adsorbent electrophoresis and chromatography separation technology, it is to point to by the traditional polymer silica, alumina, phthalein amine and a new type of adsorbent, such as polypropylene phthalein amine, sodium dodecyl sulfate ampholyte, macroporous resin as the stationary phase and the carrier, to choose a new solvent system for separation of chromatographic separation technology in the emerging development, compared with the traditional extraction chromatography chromatography separation efficiency higher, more suitable for standard into the traditional Chinese medicine and traditional Chinese medicine (TCM) physical separation of the active ingredient refined identification and analysis. Such as capillary electrophoresis, capillary zone electrophoresis. Gel electrophoresis, isoelectric focusing electrophoresis, isotachophoresis, and supercritical fluid chromatography, micellar electrokinetic capillary chromatography, fast protein liquid chromatography ( CFPLC) Such as technology, has gradually in the Chinese native medicine ingredient of widespread application in the separation, analysis and identification. Using high performance liquid chromatography (HPLC) method for the separation and analysis of flavonoids, give priority to with C18 column adsorption with adding organic regulator instead of acids to adjust PH value to suppress dissociation, can significantly reduce the composition is trailing, improve the separation efficiency of reverse high performance liquid chromatography (HPLC). In addition, Chen was quoted as saying by the new separation technologies such as reverse micelle extraction method is used to separate proteins and enzymes. Cases of some applications such as micelle electric capillary chromatography separation and determination of the great Huang Zhong emodin, rhein and the content of aloe emodin. Goes to some applications such as high-speed countercurrent chromatography separation/k two preparation time and at the same time examines mobile phase flow rate on separation efficiency and effect, show that the method can be used as a new method of preparation of drug standard. CAI a high-speed countercurrent chromatography separation are used for the prevention of such as have the main alkaloids, in chloroform under a phosphate citric acid buffer system to expand, and datura flower, sophora, three Chinese fir the separation of alkaloids, and solvent method, column chromatography, PH gradient method of the first two points after fat water step operation, compared to the method in paper chromatography optimization solvent system, with the quantitative recovery, synchronous separation, efficient fast, suitable for the separation of the advantages of large range of polarity. Xiong Mou such as use of alcohol leaching a diatomite adsorption resin separation, refined effective medicinal ingredients extracted from ginkgo biloba flavonoids, optimized the process conditions, product yield can be up to 2. 8 25%, among which the flavonoids content is higher than 2. 8%. Diterpene lactone is more than 6%, the quality is in conformity with export standards. Zhang and other application of macroporous adsorption resin to extract camptothecin, screening of resin was studied, the concentration of the extract, temperature, PH and salt concentration on the adsorption and the selection of analytical agent, the influence of PH value, the influence of velocity of parsing, the suitable adsorption and analytical conditions were determined. Luo mentioning by macroporous resin method such as separation of radix arnebiae seu lithospermi cell cultures of shikonin, in HD Type 1 best macroporous resin, and adsorption quantity is big, per milliliter of wet resin adsorption quantity can reach 8. 69 mg/ml parsing to more than 96%, compared with the traditional extraction method, can reduce the organic solvent usage more than 60 times, and saves the energy, simplify operation, significantly reduce the cost of separation, is conducive to industrialization. The progress of Chinese medicine in the process of extraction and separation of high and new technology application

vacuum freeze drying enrichment ( 真空冷冻干燥 荷兰国际集团(ing)浓度) Application of
vacuum freeze drying enrichment technology, is refers to under reduced pressure atomization, low temperature freeze sublimation, quickly remove solvent or water of a highly efficient new enrichment technology. Can rapid enrichment enrichment of effective components in extraction, low operating temperature for thermal unstable, the active ingredient is not destroyed, more suitable for the extraction and separation of effective components from the life activity of the organization, has been widely applied in biological engineering and special in the Chinese native medicine preparation process. Meng one by freeze drying method, such as deer antler and its amino acid, organic matter, iron and zinc, total nitrogen and total phosphorus content than traditional processing method, the ash content is smaller. Yang, etc. Through the study of the freeze drying experiments of straw mushrooms, samples in the process of research center and the changes of temperature and water separation with time in the process of freeze-drying quality layer and plasma layer drying of material and heat balance, the sublimation of established consistent with actual absorption analytical mathematical model, the freezing process of material provide forward-looking theoretical guidance. Tu, etc by freeze drying experiments of carrots and potatoes, on the basis of material balance and heat balance simplified calculation parameters, the mathematical model is established and verified the freezing, which can accurately predict the whole time. Sun such as green pole cool liposomes prepared by dry method, opened up a new preparations of Chinese medicine special field of application of compared with phacoemulsification, liposome frozen one with water for injection can quickly form liposome, stability is improved significantly. The progress of Chinese medicine in the process of extraction and separation of high and new technology application
with all kinds of high and new technology of modern science and technology method in continuous application in the process of extraction and separation and preparation of Chinese medicine, traditional Chinese medicine studies will certainly be developed greatly.
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