Traditional Chinese medicine extraction equipment for large ring lactone class antibiotic extraction separation
Chinese medicine extraction equipment for the large ring lactone class antibiotic extraction separation should be paid attention to avoid overly acidic or basic, acidic conditions will cause the hydroxyl on the 6th of C - Nine of carbonyl nucleophilic attack, erythromycin and dehydration. The extraction method for: at 30 degrees, pH10 with butyl acetate or pentanoic acid, concentrated than a 7:1 - - 3:1 extraction yield of 95% 98%; The second step back extraction pH4. 1 - - 5. 4, use acetate or phosphate buffer; The third step in organic solvent extraction; Then add sodium thiocyanate, erythromycin thiocyanate from solvent, further refined.
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